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His6-BaRocF assayed in the presence of various concentrations of different metals. The purified arginase samples were heat activated (50–55°C for 30 minutes) with increasing concentrations of (A) CoCl2, (B) MnSO4, or (C, D) NiCl2 at pH 9.0 (in BTP-buffered arginine). At least two experiments were conducted for each sample in triplicate. Data are presented as mean arginase activity (nmol L-orn/min/mg protein) ± standard deviation.
