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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Bone. 2008 Jan 26;42(5):861–870. doi: 10.1016/j.bone.2007.12.226

Figure 1.

Figure 1

Characterization of muBMSCs. (A) Flow cytometry assessment of immunophenotype. Representative muBMSC cultures were examined for the following surface antigen expression: CD14 (LPS receptor), CD29 (integrin β1), CD44 (hyaluronate receptor), CD45 (leukocyte common antigen), CD73 (5′ ectonucleotidase), CD90 (Thy1), CD105 (endoglin), CD117 (c-kit), Sca1 (stem cell antigen). The black lines represent the histogram determined with the antigen specific antibody while the gray lines represent the histogram determined with the isotype-matched non-specific antibody control. (B) Histochemical assessment of morphology. Primary cultured muBMSCs (passage 3) were maintained in Stromal Medium (a) or induced to undergo adipogenesis (b) or osteogenesis (c) for 6 to 9 days. Individual wells were fixed and stained for (a) fibroblasts (Toluidine Blue), (b) adipocytes (Oil Red O), or (c) mineralization (Alizarin Red).