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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Bone. 2008 Jan 26;42(5):861–870. doi: 10.1016/j.bone.2007.12.226

Figure 2.

Figure 2

Expression profile of mRNAs encoding transcriptional regulatory factors relating to the core circadian oscillator following dexamethasone exposure of muBMSCs. Confluent, quiescent cultures of muBMSCs isolated from FEB female mice were exposed to 1 μM dexamethasone for 2 hrs and subsequently harvested at 4 hr intervals for isolation of total RNA. Real time PCR analysis was performed to examine expression of mRNAs Albumin D binding protein (DBP), Brain-Muscle Arnt-Like 1 (bmal1), Cryptochrome 1 (cry1), Period 1 (per1), Period 3 (per3), Rev-erb α., and Rev-erb β, all normalized relative to the control, cyclophilin B. Assays were preformed in triplicate and display the mean ± S.D.