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Frequency of reliable and significant 17-mer MPSS signatures in a subset of 5'-to-3' oriented contigs. Signatures were mapped based on their location relative to the 3' end of the EST contigs. Most signatures were found at the 3'-most DpnII site, indicated as position #1 on the x-axis. However, expressed MPSS signatures were found as far 5' as eighth DpnII site from the 3' end of the contig.
