Table 1.
A. Summary of sequencing results. | ||||||||
17-base signatures | 20-base signatures | |||||||
Sequencing frame | Absolute | % of Total | Absolute | % of Total | ||||
2-step run | 1,194,288 | 45.3% | 1,002,346 | 44.4% | ||||
4-step run | 1,441,005 | 54.7% | 1,256,940 | 55.6% | ||||
B. Single filter results. | ||||||||
17-base signatures | 20-base signatures | |||||||
Reliability Filtera | Count | Proportion | Count | Proportion | ||||
R | 17,976 | 58.5% | 15,699 | 58.4% | ||||
nR | 12,761 | 41.5% | 11,179 | 41.6% | ||||
Significance Filtera | ||||||||
S | 16,029 | 52.1% | 13,817 | 51.4% | ||||
nS | 14,708 | 47.9% | 13,061 | 48.6% | ||||
C. Combined filter results. | ||||||||
17-base Total | 17-base Distinct | 20-base Total | 20-base Distinct | |||||
Filter categoriesa | Absolute | % of Total | Count | % of Total | Absolute | % of Total | Count | % of Total |
RS | 2,550,024 | 96.8% | 13,586 | 44.2% | 2,187,369 | 96.8% | 11,874 | 44.2% |
RnS | 17,862 | 0.7% | 4,390 | 14.3% | 14,958 | 0.7% | 3,825 | 14.2% |
nRS | 47,346 | 1.7% | 2443 | 7.9% | 39,338 | 1.7% | 1943 | 7.2% |
nRnS | 20,061 | 0.8% | 10,318 | 33.6% | 17,621 | 0.8% | 9,236 | 34.4% |
TOTAL | 2,635,293 | 100.0% | 30,737 | 100.0% | 2,259,286 | 100.0% | 26,878 | 100.0% |
"Absolute" indicates the total number of successful sequencing reads, while "count" or "distinct" indicates the number of different sequences.
a R = reliable, nR = non-reliable, S = significant, nS = non-significant, RS = reliable and significant, RnS = reliable and non-significant, nRS = non-reliable and significant, nRnS = non-reliable and non-significant.