Table 3.
Matched and un-matched Vitis EST contig and singletons.
| A. EST contigs. | ||||
| 17-mer | 20-mer | |||
| Categorya | Count | Freq | Count | Freq |
| 5' and 3' | 942 | 6.4% | 736 | 5.0% |
| 5' or 3' | 5,488 | 37.4% | 5,095 | 34.8% |
| Subtotal | 6,430 | 43.9% | 5,831 | 39.8% |
| With tag but no match | 6,875 | 46.9% | 7,474 | 51.0% |
| Without tags | 1,353 | 9.2% | 1,353 | 9.2% |
| Subtotal | 8,228 | 56.1% | 8,827 | 60.2% |
| Total | 14,658 | 100.0% | 14,658 | 100.0% |
| B. EST singletons. | ||||
| 17-mer | 20-mer | |||
| Categorya | Count | Freq | Count | Freq |
| 5' and 3' | 238 | 1.6% | 263 | 1.8% |
| 5' or 3' | 1,952 | 13.3% | 1,834 | 12.5% |
| Subtotal | 2,190 | 14.9% | 2,097 | 14.3% |
| With tag but no match | 9,074 | 61.8% | 9,167 | 62.4% |
| Without tags | 3,667 | 25.0% | 3,667 | 25.0% |
| Subtotal | 12,741 | 86.8% | 12,834 | 87.4% |
| Total | 14,931 | 100.0% | 14,931 | 100.0% |
a Categories are as follows: "5' and 3"' indicates contigs matched by reliable and significant (RS) tags in both sense and antisense orientation simultaneously; "5' or 3"' indicates contigs matched in their sense or antisense orientation but not both; "with tag but no match" indicates contigs with in silico identified GATC tag sites but without sequenced signatures (the presence of a DpnII site but no matching MPSS tag, see materials and methods for details); and "without tags" indicates contigs lacking GATC sites.