Table 4.
Grape ESTs derived from distinct tissue types matched by MPSS signatures (only from Vitis vinifera).
| A. EST contigs. | |
| Categories | Contigs |
| Berry pre-veraison | 79 |
| Berry veraison | 117 |
| Berry post-veraison | 359 |
| Subtotal berry | 555 |
| Compound bud | 87 |
| Flower | 132 |
| Seed | 1 |
| Petiole | 63 |
| Stem | 67 |
| Leaf | 332 |
| Roots | 5 |
| Subtotal from single organ library (excluding berry libraries) | 687 |
| Subtotal from single organ librarya | 1,242 |
| 2-Organs | 1,520 |
| 3-Organs | 1,084 |
| 4-Organs | 763 |
| 5-Organs | 491 |
| 6-Organs | 312 |
| 7-Organs | 181 |
| 8-Organs | 135 |
| 9-Organs | 46 |
| All 10 sampled organs | 10 |
| Subtotal from multiple organ librariesb | 4,542 |
| Subtotal non-ESTs | 6 |
| Total matched contigs and non-EST sequences | 5,791 |
| Total matched contigs not previously associated with any berry libraryc | 1,038 |
| Total matched contigs previously associated with berry librariesf | 4,753 |
| A. Total matched contigs from other grape berry librariesd | 3,439 |
| B. Total matched contigs with ≥1 EST derived from stage II berry librariese | 1,314 |
| Total unmatched contigs and non-EST sequences | 7,567 |
| C. Total unmatched contigs from all libraries except (D) | 6,990 |
| D. Total unmatched contigs corresponding to UCD stage II berry librariesh | 577 |
| B. EST singletons. | |
| Categories | Contigs |
| Berry pre-veraison | 177 |
| Berry veraison | 343 |
| Berry post-veraison | 389 |
| Subtotal berry | 909 |
| Compound bud | 45 |
| Flower | 231 |
| Seed | 16 |
| Petiole | 34 |
| Stem | 89 |
| Leaf | 414 |
| Roots | 49 |
| Subtotal from other libraries (excluding berry libraries) | 878 |
| Total matched singletons | 1,787 |
| A. Total matched singletons with ≥ 1 EST derived from stage II berry libraries | 207 |
| Total unmatched singletons | 9,754 |
| B. Total unmatched singletons from all libraries except (D) | 8,947 |
| C. Total unmatched singletons corresponding to UCD stage II berry libraries | 807 |
Only 7,686 distinct, reliable and significant, 17-base MPSS signatures were used in this analysis.
a Matched contigs with at least one EST derived from berry libraries excluding those from UCD. Berry libraries in this category include multiple developmental stages and growing conditions [4].
b Matched contigs with ESTs found in cDNA libraries originated from at least two or more different grapevine organs. It may or may not simultaneously include berry libraries.
cIndicates matched contigs assembled with ESTs not previously associated with berry libraries
dIndicates matched contigs assembled with ESTs not previously associated with the UCD flower-berry libraries. This number includes contigs with at least one EST derived from flower-berry libraries reported by other groups.
e Indicates contigs containing at least one EST produced from the flower-berry UCD libraries [4].
fCorresponds to the sum of A and B.
gTotal number of contigs and non-ESTs sequences [4] not matched by 17-mer reliable and significant tags. This value corresponds to the sum of C and D.
h Contigs with at least one EST derived from SII berry libraries [4] but not matched by 17-mer reliable and significant tags.