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Construct of a typical short-hairpin RNA (shRNA) vector insert, 5′–3′. Different restriction sequences are placed on the 5′ and 3′ ends. A 19 bp sequence for the target mRNA (sense sequence), 9 bp stem loop, and a 19 bp reverse complementary of the target sequence. When transcribed, the insert will form a secondary hairpin structure to cause the knockdown of the target gene.
