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. Author manuscript; available in PMC: 2008 Jun 9.
Published in final edited form as: Methods Mol Biol. 2007;371:9–19. doi: 10.1007/978-1-59745-361-5_2

Table 1.

Culture Media Components of Common Cell Types

Cell line Base media Supplements Serum
MRC-5, WI-38, IMR-90 fetal lung fibroblasts, BJ foreskin fibroblasts Minimum Essential Medium (MEM) Eagle + Earle's Basic Salt Solution (BSS) 2X concentration of essential & nonessential amino acids and vitamins 2 mM l-glutamine, 1.5 g/L sodium bicarbonate, 1.0 mM sodium pyruvate 15% FBS, uninactivated
CCL-39 Chinese hamster lung fibroblasts McCoy's 5a 10% FBS
A9 L mouse fibroblasts Dulbecco's Minimum Essential Medium (DMEM) 4 mM l-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose 10% FBS
C2C12 mouse myoblasts Dulbecco's Minimum Essential Medium (DMEM) 4 mM l-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose 10% FBS (or 10% horse serum to enhance myotube formation)
RBL-2H3 rat basophilic leukemia Minimum Essential Medium (MEM) Eagle + Earle's Basic Salt Solution (BSS) 2 mM l-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM nonessential amino acids, 1.0 mM sodium pyruvate 15% FBS, heat-inactivated