FIGURE 1.

cys-LT receptor expression and calcium signaling by LTD₄ and LTE₄ in LAD2 cells. A, dose-dependent effects of LTC₄, LTD₄, and LTE₄ on the accumulation of intracellular calcium in LAD2 cells. LAD2 cells were loaded with Fura-2-AM and stimulated with the indicated concentrations of LTs in various orders. B, effect of treatment with MK571 (1 μm, 5 min) on calcium flux by Fura-2-AM-loaded LAD2 cells stimulated with the indicated LTs (500 nm each). C, real-time PCR analysis of CysLT₁R, CysLT₂R, and GPR17 transcripts expressed by LAD2 cells. D, flow cytometry analysis of CysLT₁R, CysLT₂R, and GPR17 proteins. CysLT₁R and CysLT₂R were detected after permeabilization of LAD2 cells and staining with anti-C terminus Abs. GPR17 staining was performed with an anti-N terminus Ab both with (+perm) and without (–perm) permeabilization. Shaded curves are staining with nonspecific rabbit IgG. Results depicted are from single experiments, representative of at least three performed for each assay.