Cytokine mRNA induction and MIP-1β generation by LAD2 cells
stimulated with cys-LTs. A, real-time PCR showing relative levels
of MIP-1β, MCP-1, IL-5, IL-8, and TNFα transcript expression by
LAD2 cells stimulated with 100 nm LTD4 or
LTE4 for 2 h. Data are mean ± S.E. from three experiments.
B, dose-dependent effect of LTE4 on MIP-1β secretion
by LAD2 cells stimulated for 6 h with the indicated concentrations of cys-LTs.
Results are mean ± S.E. from three experiments. C, MIP-1β
concentrations were measured in supernatants collected from cells after 6 h of
stimulation with LTD4 (100 nm), LTE4 (100
nm), or anti-IgE (1 μg/ml) as a positive control. D,
effect of pretreatment with MK571 (1 μm) on MIP-1β
generation by cells stimulated with LTD4 (100 nm), or
LTE4 (100 nm) for 6 h. Results are expressed as the mean
± S.D. from the three experiments performed. * and ** indicate
p < 0.05 and <0.01, respectively, relative to the control in
B and C and relative to the samples without MK571 in
D.