FIGURE 7.

Effect of LTD₄ and LTE₄ on PPARγ-dependent ERK activation and PGD₂ generation by primary hMCs. A, cord blood-derived hMCs (6-week-old) were stimulated for 10 min with LTD₄ or LTE₄ (500 nm) in the absence or presence of GW9662 (10 μm) or MK571 (1 μm). Results are from a single experiment representative of three separate experiments performed. B, PGD₂ generation induced by stimulation of primary hMCs with LTD₄ or LTE₄ at the indicated concentrations. Results are the mean ± ½ range for two experiments. C, effects of GW9662 (10 μm) or MK571 (1 μm) on cys-LT-induced PGD₂ generation by primary hMCs. Cells were stimulated with 100 nm of the indicated cys-LT for 6 h. Results were measured with the PGD₂-MOX assay and are the mean ± S.E. of three independent experiments. * indicates p < 0.05.