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. 2008 Jun 13;283(24):16355–16362. doi: 10.1074/jbc.M708985200

FIGURE 4.

FIGURE 4.

Time courses of inactivation of M662C/D1828C FVIIIa variants by APC and protein S (PS) or FV. Activity of thrombin-activated FVIIIa variants was monitored over time by FXase assay. A, FVIIIa with 2.5 nm APC. B, FVIIIa with 2.5 nm APC and 100 nm protein S. Inset, FVIIIa with 2.5 nm APC and 12 nm FV. ▵, ▴, M662C/D1828C; ○, •, M662C/D1828C/R336Q; □, ▪, M662C/D1828C/R562Q. Two to three experiments were averaged together. M662C/D1828C inactivation was fit to a double exponential decay. M662C/D1828C/R336Q and M662C/D1828C/R562Q were fit to single exponential decay curves. Standard deviations are shown as error bars.