TABLE 2.

Primers used in this study (classified) All oligonucleotides were purchased from IDT (Integrated DNA Technologies).

Primer No.	Sequence
Construction of deletion mutants
ΔphoP
399	tgt tct tat tgt taa cac aag gga gaa gag cat atg aat atc ctc ctt ag
400	gcg gca gaa aat ggc gag caa att tat tca gtg tag gct gga gct gct tc
ΔslyA
11	ata ata act tag caa gctaat tat aag gag cat atg aat atc ctc ctt ag
12	gtc aca tgg cca cac gta tgc ccc tgc acc gtg tag gct gga gct gct tc
Δhns
350	cgg cgg gat ttt aag cat cca gga agt aaa gtg tag gct gga gct gct tc
351	cca ccc caa tat aag ttt gag att act aca cat atg aat atc ctc ctt ag
Δup-phoP up-6
395	tcg acg aac tta aat aat gcc tgc ctc acc cat atg aat atc ctc ctt ag
396	aaa tag tca ccc tct ttc tga aga aaa gag gtg tag gct gga gct gct tc
575	ttt ggg gat aaa cag tta ata aac cag aca tta tca cac cctt ctt tct gaa gaa aag
Construction of chromosomal HA epitope in hns locus
350	cgg cgg gat ttt aag cat cca gga agt aaa gtg tag gct gga gct gct tc
352	aag caa ctg gaa gat ttc ctg atc aag gaa tat ccg tat gat gtt cct gat tat gct taa cat atg aat atc ctc att ag
Construction of chromosomal lacZ fusion
pcgL
38	ttc ccg tta agc aga gac cct gaa acg gcg cat atg aat atc ctc ctt ag
39	cag gcg taa tga tta gat ttc gca aca aaa gtg tag gct gga gct gct tc
pagC
130	ggc ttc aac gtc ggg gtt gga tac cgt ttc gac tac aag gac gac gat gac aag taa cat atg aat atc ctc ctt ag
131	aag gcg aac ctt ccg cat agc tta tgc ttt gtg tag gct gga gct gct tc
Construction of plasmids
pYS1000
1	gcg tcg act tta cac ttt aag ctt ttt atg ttt atg ttg tgt gga act gca gcc gct cga gca gga aac agc tat gac cat gat tac g
2	ccc aag ctt aaa aaa aac cgg gca ttg ccc ggt ttt ttt aat gga ttt cct tac gcg aaa tac ggg c
pYS1100
409	gcg tcg acg aac tta aat aat gcc
410	ccg ctc gag tag cgt tga tta tgg tgc
pYS1115
538	ctc ttt tct tca gaa aga ggg tgt gat aat gtc tgg ttt att aac
539	cac cct ctt tct gaa gaa aag agg gtg agg cag
pYS1118
595	cgg gat cca gtt tga gat tac tac aat gag cg
594	ccc aag ctt aat att cct tga tca gga aat c
pYS1119
595	cgg gat cca gtt tga gat tac tac aat gag cg
596	ccc aag ctt aag cat aat cag gaa cat cat acg gat att cct tga tca gga aat c
pYS1244
775	aat aat gcc tgc ctc acc ctc ata tga aca gaa aga ggg tga cta t
776	gag ggt gag gca ggc att att taa gtt cgt cga
Reverse transcription-PCR
phoP
223	atg atg cgc gta ctg gtt g
226	gta gta atc agc ttc cct g
pcgL
580	atg gta aaa tgc gta tct tc
581	caa agt ctt ttg gct ggt g
pagK
582	atg aaa cac gtc aag agc
583	tta tga tct tga gag tct g
phoN
578	atg aaa agt cgt tat tta g
579	tca ctg att ctt cag gag
pagC
584	atg aaa aat att att tta tcc
585	ctt tgt gca tac ccc acg g
ugtL
586	atg aag aaa tca gat ggt g
587	gta aca gca ata ata gcc
rpoD
346	cca tct gcc gga aga tat cg
347	cgg cat cag gcg ctt ctt cc
ChIP assay
phoP promoter
409	gcg tcg acg aac tta aat aat gcc
410	ccg ctc gag tag cgt tga tta tgg tgc
pcgL promoter
675	aca cat cgt tat ctg tgc
676	tgt tac acc tcg cga gag
pagK promoter
673	tgt taa ctg gcc tgt tcc
674	tac ctg tta aat tgt ggc
phoN promoter
677	aga ttt taa cct aat gcg
678	aga ctc act ccg gat cag
pagC promoter
505	tgg aac gtc att gac
506	ttt att ccc gct ccg
EMSA assay and footprinting assay
phoP promoter
469	cgc cgg caa att ata tcg gtc
470	ggt gtt cat taa ggt agt aat c
pcgL promoter
675	aca cat cgt tat ctg tgc
676	tgt tac acc tcg cga gag
pagK promoter
673	tgt taa ctg gcc tgt tcc
674	tac ctg tta aat tgt ggc
phoN promoter
677	aga ttt taa cct aat gcg
678	aga ctc act ccg gat cag
pagC promoter
513	ata atg act tgt gaa gtt c
514	cta tca gtt aca aca ttt c