FIGURE 4.

Role of p300 in eNOS transcription in response to laminar shear. A shows effect of the p300 inhibitor curcumin on eNOS transcription in response to 6 h of shear (n = 4–5/group). B shows effect of p300 siRNA on p300 protein levels as determined by Western blot. As evident, p300 siRNA effectively reduced p300 levels whereas control (Scrambled) siRNA did not. Down-regulation of p300 with siRNA prevented acetylation of p65 as shown in the example in C and in the mean data (n = 4) presented in D. Inhibition of p300 expression with siRNA completely prevented the increase in eNOS mRNA as determined by real-time quantitative PCR (D, n = 4–5). Data are mean ± S.E. of the mean.