FIGURE 4.

Inhibition of anti-gp41 CT antibodies on HIV-1 Env-mediated cell-cell fusion at suboptimal temperature. CHO-WT cells and 3T3-T4-CXCR4 cells were cocultured for 6 h in the presence of LLP1–2-, LLP1-, LLP2-specific IgG, anti-LLP1–2 IgG/LLP2, and normal rabbit IgG (control), respectively, at graded concentrations at 31.5 °C (A) or 37 °C (B) and then cocultured for additional 16 h at 37 °C. The syncytia were then counted under a microscope. The data represent triplicate determinations that were performed at least three times. All results are presented as mean ± S.D.