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Base-catalyzed Kemp elimination of 5-nitrobenzisoxazole (1). The base abstracts the C-3 proton from the substrate (1) and induces its decomposition. Antibody 34E4 was raised against hapten 4 and efficiently catalyzes the Kemp elimination. Compound 5 is a hapten derivative, which was used for measuring the hapten binding kinetics, and compound 6 is a noncleavable substrate analog, which served as a model compound to determine the substrate binding kinetics.
