FIGURE 6.

Localization of TgIF2K-A and TgIF2K-B. A, IFA were performed on a transgenic parasite clone expressing GFP fused to the ER retention signal, HDEL (15). Intracellular (IC) or extracellular (EC) GFP-HDEL parasites were co-stained with affinity-purified anti-TgIF2K-A (green), anti-GFP (red), and 4′,6-diamidino-2-phenylindole (DAPI; blue). B, IFA performed on wild-type parasites using anti-TgIF2K-B (green) and 4′,6-diamidino-2-phenylindole (red) shows diffuse staining of parasite cytoplasm in intracellular (IC) and extracellular (EC) parasites.