Skip to main content
. 2008 Jun 13;283(24):16514–16524. doi: 10.1074/jbc.M708177200

FIGURE 4.

FIGURE 4.

Orlistat inhibits AMPK activation without affecting cAMP levels and PKA signaling. A, intracellular levels of cAMP in 3T3-L1 adipocytes treated with forskolin with/without orlistat as described in Fig. 2. B, cell lysates obtained in the experiment described in Fig. 2 were immunoblotted (IB) with antibodies recognizing phosphorylated serine and threonine residues of PKA substrates (∼P-PKA), totalα-AMPK, the PKA downstream targets P-LKB1 Ser-431, P-CREB Ser-133, and β-actin. Densitometric analyses are as follows: C, P-PKA substrate ∼62 kDa; D, α-AMPK; E, P-LKB1 Ser-431; and F, P-CREB Ser-133. Immunoblots shown are representative of nine samples obtained in three independent experiments. Results in A, C, D, E, and F are expressed as means ± S.E. (n = 9). Significantly different from control group: ***, p < 0.001. Iso, isoproterenol.