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. 2008 Jun 13;283(24):16514–16524. doi: 10.1074/jbc.M708177200

FIGURE 5.

FIGURE 5.

The absence of perilipin or the shRNA-mediated silencing of ATGL inhibit both forskolin-stimulated lipolysis and AMPK activation in Peri-/- MEF adipocytes. Adipocytes retrovirally engineered from murine embryonic fibroblasts of perilipin null mice (Peri-/- MEFs) were transduced with an adenovirus expressing GFP (negative control) or wild-type perilipin A (WT Peri A) and with either an ATGL-directed shRNA or a scrambled shRNA. After differentiation to adipocytes, cells were serum-depleted in DMEM containing 2% fatty acid-free BSA and treated for 2 h with/without forskolin. A, medium glycerol; B, medium FFA. Representative immunoblots of phosphorylated serine and threonine residues of PKA substrates (∼P-PKA), perilipin, ATGL, P-AMPK Thr-172, α-AMPK, and β-actin (C). Densitometric analysis of P-AMPK Thr-172 (D) and α-AMPK (E) immunoblots. Results in A and B are expressed as means ± S.E. (n = 9) obtained in three independent experiments. Significantly different from basal control group: *, p < 0.05, ***, p < 0.001. IB, immunoblot.