FIGURE 3.

LPA increased HDAC enzyme activity. A, I-83 cells were treated with LPA in various concentrations. 10 μg of nuclear extract was assayed for HDAC enzyme activity as described under “Experimental Procedures.” CaOv-3 (B), BJAB, I-83, and HEK293 (C), and primary CLL cells (D) were treated with LPA at a concentration 10 μm for as long as 2-h time course in serum-free medium. 10 μg of nuclear extract was assayed for HDAC enzyme activity as described under “Experimental Procedures.” Error bars represent the standard deviations for three independent experiments.