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. 2008 Feb 25;28(11):3713–3728. doi: 10.1128/MCB.02263-07

FIG. 3.

FIG. 3.

The search for a Kcnq1ot1 fragment with bidirectional silencing features over a 52-kb portion of the Kcnq1ot1 transcription unit revealed none of the fragments that have similarities with the Kcnq1ot1 SD. (A) Physical maps depicting the Kcnq1/Kcnq1ot1 locus, overlapping fragments of 3 to 5 kb of the Kcnq1ot1 transcription unit used in episome-silencing assays, and a diagrammatic presentation of the cloning of the Kcnq1ot1 gene fragments into the PS6A1 episome. (B) Bar graph shows the percentage activity of the hygromycin gene in PS6A1Kcnq1ot1Frg1 to -15, analyzed by counting the hygromycin-resistant cell colonies. The data represent means ± standard deviations for a minimum of three independent experiments.