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. 2008 Feb 25;28(11):3713–3728. doi: 10.1128/MCB.02263-07

FIG. 4.

FIG. 4.

High-affinity chromatin attachment regions of the Xist transcript increase the efficiency of bidirectional silencing. (A) A schematic representation of the Xist RNA. The silencing domain containing the A-rich repeats is located at the 5′ end of the transcript. The line labeled xc4.4 indicates the chromatin localizing region that has previously been mapped (39). (B and C) Schematic maps depicted alongside the bar graphs are the wild-type and mutant Kcnq1 ICR fragments (the Kcnq1ot1 SD is either flanked with a high-affinity Xist chromatin localizing region or replaced with the Xist SD) inserted into the PS6 or PS6A1 episome. The schematic map of the PS4ΔH19 polyA9.2 construct alongside the bar graph is shown along with the reporter genes. PS6Xist SD+ and PS6Xist SD- indicate that the Xist SD was inserted in both positive and negative orientations, respectively, in PS6A1. Bar graphs show the percentage activities of the hygromycin and H19 genes in the episomes containing wild-type and mutant Kcnq1 ICR fragments, calculated as described in the legend for Fig. 2. The data represent means ± standard deviations for three independent experiments.