FIG. 5.

The Kcnq1ot1 SD mediates transcriptional silencing through regulating the chromatin structure. Quantitative analysis of H3K9Me3 and H3K9Ac profiles using ChIP over the H19 and hygromycin gene promoters on cross-linked chromatin obtained from JEG-3 cells transfected with the PS6, PH19, or PS6A1 episomes. Bar graphs shows percent enrichment, and the data were quantified as described in Materials and Methods. The data represent means ± standard deviations for three independent ChIP experiments.