FIG. 5.

Mapping the exonic regulatory elements within the BRCA1 exon 18 sequence. (A) Schematic representation of the BRCA1 exon 18 deletion mutants. The G or T substitution at position +6 and the deleted nucleotides are indicated, while the TAG motives are underlined. (B and C) Exon 18 WT (B) and T6 (C) serial deletion analysis. The indicated minigenes were transfected into Hep3B cells, and the splicing pattern was analyzed by RT-PCR. The two resulting splicing products corresponding to exon 18 inclusion (ex18+) and exclusion (ex18−) are indicated, and the percentage of exon inclusion is reported. (D) Overexpression of ASF/SF2 in T6 deletion mutant minigenes. The indicated constructs were cotransfected with the ASF/SF2 coding plasmid (+) or with the empty vector (−).