Figure 2.

Induction of Myc expression is followed by repression of gas1 mRNA expression. Rat-1 cells were made quiescent by growing to confluence followed by serum-deprivation in 0.1% FBS/DMEM for 72 hr, and then were stimulated with serum (20% FBS, final concentration) for the indicated times. RNA underwent Northern blot analysis for gas1 and c-myc expression. The 28S rRNA band in the ethidium bromide stained gel is shown as a control for RNA loading. Hybridization signals were quantitated by using nih image software.