Figure 3.

Myc represses gas1 promoter activity. (A) Schematic representation of c-Myc and mutants used. (B) Gas1 and ODC reporter plasmids (14, 33) were cotransfected into Rat-1 cells together with the c-myc expression vector pCMV-Hm (32), or with plasmids expressing the MB2 or LZ deletion mutants of myc, or with empty vector. All transfections included a pCMV-β-galactosidase plasmid to normalize for transfection efficiency. (C) Western blot analysis of wild-type and mutant Myc proteins following transient transfection.