Figure 5.
Rate of gas1 gene transcription following activation of MycER. (A) Rat1/MycER cells were made quiescent as described in Figs. 2 and 4, and treated for 0, 1, and 3 hr with 4-OHT (200 nM). Nuclei were prepared and nuclear run-off transcription assays were performed as described in Materials and Methods. 32P-labeled transcripts were hybridized to DNA probes immobilized on nitrocellulose membranes. Blots were visualized by autoradiography. (B) Quantitation of blots was performed by using hih image software. gas1 band intensities were normalized to that of gapdh, and the amounts relative to that at 0 hr are shown.