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. 2008 Mar 24;28(11):3652–3662. doi: 10.1128/MCB.01923-07

FIG. 4.

FIG. 4.

Live-cell time-lapse microscopy of Hice1-depleted cells. (A) Top row (control siLuc treated), a cell with normal mitotic progression; bottom row (siHice1 treated), a representative cell that exhibited early tripolar spindle formation (<20 min after entering mitosis). (B) A representative cell (siHice1 treated) displaying an additional small half-spindle (MT aster) at ∼3 h after entering mitosis. The aster underwent gradual but progressive growth over time and eventually gave rise to a tripolar structure. (C) An Hice1 siRNA-treated cell with a dynamically unstable and abnormal spindle (arrowheads), which appeared as a near-bipolar spindle with fragmented poles (time points 0:40 to 1:20), later became a near-bipolar structure with a single extra half-spindle (time points 1:40 to 2:20), and then returned to a near-bipolar one with fragmented poles (time point 3:20). Bars, 10 μm.