FIG. 2.

PLCγ2 expression is stable during osteoclast differentiation, and PLCγ2 is localized to the actin ring in the osteoclast. (A) WT and PLCγ2^−/− BMDM were grown in the presence of 10 ng/ml M-CSF and 100 ng/ml RANKL for 5 days. Whole-cell lysates were collected every 24 h and Western blotted (WB) for the indicated proteins. (B) WT and PLCγ2^−/− BMDM were retrovirally infected with pMX (empty vector) (top row) or pMX-NFAT2 (bottom two rows). Osteoclasts were probed with PLCγ2 polyclonal antibody and costained with Cy3-phalloidin and DAPI. Images were obtained with an Olympus IX70 inverted fluorescence microscope using a 60× APO, 1.4-numerical-aperture lens. Scale bar, 5 μm.