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. 2008 Mar 31;28(11):3610–3622. doi: 10.1128/MCB.00259-08

FIG. 5.

FIG. 5.

Protein-protein interactions between components of the integrin adhesion complex are decreased in PLCγ2−/− cells. (A, B) WT and PLCγ2−/− preOCs were starved for 6 h and stimulated by adhesion to vitronectin for various times. Lysates were collected, normalized, and immunoprecipitated with a Src monoclonal antibody (A) or a PYK2 monoclonal antibody (B). Immunoprecipitates were Western blotted (WB) for the indicated proteins. (C) Whole-total cell input lysates (TCL) were Western blotted for the indicated proteins. Equal amounts of proteins were loaded in each lane. (D) WT adherent osteoclasts were treated with U73122 (5 μM) for 3 h or left untreated. Lysates were immunoprecipitated with a Src monoclonal antibody and Western blotted for the indicated proteins. The input lysates (TCL) were Western blotted for the same proteins. Equal amounts of proteins were loaded in each lane.