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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Sep 5;226(3):213–224. doi: 10.1016/j.taap.2007.08.024

Fig. 1.

Fig. 1

Time course of the inductive effects of BKF exposure on E2 metabolism in T-47 D cells. Confluent cultures of T-47D cells in 6-well plates were exposed to a single application of medium containing 3 μM BKF (2 ml/well). At the indicated times, the medium was replaced with medium containing 1 μM E2 with 0.1% DMSO as the vehicle. After a 6-h assay period, media were recovered, conjugates were hydrolyzed, and rates of 2- and 4-MeOE2 formation were determined relative to cellular protein content, as described in Materials and Methods. Data are the mean ± SE of triplicate determinations. Rates of 2- and 4-MeOE2 formation for control cultures (without BKF exposure) were 0.30 + 0.02 and 0.14 + 0.02 pmol(h)−1(mg protein)−1, respectively.