Skip to main content
. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Sep 5;226(3):213–224. doi: 10.1016/j.taap.2007.08.024

Fig. 9.

Fig. 9

Kinetics of BKF hydroxylation catalyzed by human CYP1A1 and CYP1B1. Reaction mixtures containing 2 pmol of cDNA-expressed human CYP1A1 or CYP1B1 were incubated with 0.1, 0.5, 1, 2, 5 or 10 μM BKF. The metabolites produced were analyzed by HPLC with fluorescence detection as described in Materials and Methods. Values shown are the average of duplicate determinations for rate formation of 3-, 8-, and 9-OHBKF. Curves represent best fits to the Michaelis-Menten equation.