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. 2008 May 21;7:88. doi: 10.1186/1475-2875-7-88

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Copyright © 2008 Ocaña-Morgner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time-lapse microscopic analysis of the interaction between DCs and T cells after pre-incubation with P. yoelii-infected erythrocytes. DCs were differentiated in vitro and pre-incubated with uninfected (A) or P. yoelii-infected (B) erythrocytes before loading with OVA peptide 323–339. Naïve DO11.10 T cells that are specific for this OVA epitope were isolated from transgenic mice and added to DCs. Individual pictures frames from movies (Additional file 1 and Additional file 2) showing DC-T cell interactions. Arrows indicate contacts between DCs and T cells. Time in min is indicated in each frame. Representative results from one of five independent experiments are shown.