Table 1.

Primary antibodies and enzymes used in this study

Primary antibody	Specificity	Enzymatic pretreatment	Working dilution
Polyclonal*	Anti-type I collagen (whole molecule)	–	1:500
Polyclonal*	Anti-type III collagen (whole molecule)	–	1:500
Polyclonal LF-113†	Anti-decorin (IIPYDPDNPLISMC-[LPH])	0.2U Chondroitinase ABC‡	1:1000
Polyclonal LF-106†	Anti-biglycan (VPDLDSVTPTFSAMS-[LPH])	0.2 U Chondroitinase ABC‡	1:250
Polyclonal*	Anti-fibronectin (whole molecule)	–	1:250
Polyclonal*	Anti-laminin (whole molecule)	0.002% Trypsin§	1:250

^*Chemicon International, Temecula, CA, USA.

^†Dr Larry Fisher (National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA).

^‡Seikagaku Corp., Tokyo, Japan.

^§Merck, Darmstadt, Germany.