Table 1.

Analysis of intra-observer manual measurements

Pair readings/patterns	Mean difference	SD	95% CI of difference	P-value*
Observer 1: reading 1 vs. reading 2
Total	–22.20	27.461	–26.07 to –18.33	< 0.0001
Membrane	–30.18	31.352	–36.40 to –23.96	< 0.0001
Cytoplasm	–16.15	18.390	–20.67 to–11.63	< 0.0001
Nuclear	–8.90	21.710	–17.01 to –0.79	0.033
Observer 2: reading 1 vs. reading 2
Total	–9.25	18.094	–11.80 to –6.70	< 0.0001
Membrane	–10.54	23.561	–15.21 to –5.87	< 0.0001
Cytoplasm	–11.05	9.691	–13.43 to –8.66	< 0.0001
Nuclear	–1.00	3.543	–2.32 to 0.32	0.133
Observer 3: reading 1 vs. reading 2
Total	3.46	28.000	–0.49 to 7.40	0.085
Membrane	0.97	32.966	–5.57 to 7.51	0.769
Cytoplasm	8.79	25.411	2.54 to 15.03	0.007
Nuclear	0.03	4.064	–1.48 to 1.55	0.964

^*Paired t-test. CI, confidence interval.