Table 2.

Analysis of inter-observer manual measurements

Pair observers/patterns	Mean difference	SD	95% CI of difference	P-value*
Observer 1 vs. Observer 2
Total	4.85	22.605	1.67 to 8.04	0.003
Membrane	12.94	28.581	7.27 to 18.61	< 0.0001
Cytoplasm	–4.90	7.707	–6.80 to –3.01	0.000
Nuclear	–0.65	6.382	–3.03 to 1.73	0.581
Observer 1 vs. Observer 3
Total	–0.91	17.731	–3.41 to 1.59	0.473
Membrane	1.68	20.729	–2.44 to 5.79	0.421
Cytoplasm	–3.30	15.463	–7.10 to 0.50	0.087
Nuclear	–4.27	7.967	–7.24 to –1.29	0.006
Observer 2 vs. Observer 3
Total	–5.76	25.854	–9.40 to –2.12	0.002
Membrane	–11.27	33.377	–17.89 to –4.64	0.001
Cytoplasm	1.60	13.902	–1.82 to 5.02	0.354
Nuclear	–3.62	5.104	–5.52 to –1.71	0.001

^*Paired t-test. CI, confidence interval.