Fig. 1.

Interaction domain analysis. (A) Bves and GEFT truncations were generated by using PCR for further definition of interacting domains. By using a yeast two-hybrid strategy, Bves truncations (black bars) were screened against full-length GEFT (amino acids 34–618) for interaction. GEFT truncations (gray bars) were screened against the intracellular C terminus (amino acids 118–358) of Bves. Results of matings listed on right: + signifies growth on selective media, whereas − indicates no growth observed upon mating. (B) Minimal interaction domain of Bves (black bar) contains the Popdc domain, whereas minimal interaction domain of GEFT (gray bar) contains the Dbl homology domain.