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. 2008 Jun 9;105(24):8298–8303. doi: 10.1073/pnas.0802345105

Fig. 3.

Fig. 3.

GST-Bves pulldown of GEFT. GST-Bves fusion proteins representing the extracellular N terminus and cytoplasmic C terminus of Bves were tested for interaction with GEFT-GFP and NudeL-GFP. Representative mobilities of mGEFT-GFP and mNudeL-GFP are provided (lanes 1 and 2). No reactivity is observed in lanes containing isolates from GST/mGEFT-GFP (lane 3), GST-mBves1–22/mGEFT-GFP (lane 4), or GST-mBves 115–358/mNudeL-GFP pulldowns (lane 6), indicating that these proteins do not interact. A band representing mGEFT-GFP is clearly seen in the lane containing isolate from the GST-mBves115–358/mGEFT-GFP pulldown (lane 5).