Fig. 5.

Transfection of Bves-CT reduces motility and increases roundness of NIH 3T3 cells. (A) Upon transfection of Bves-CT, a significant reduction in motility speed (white bar) is observed in comparison to cells transfected with a GFP only expressing plasmid (gray bar) (Raw data are given as Movie S1 and Movie S2). (B) Roundness of cells measured in real-time as described in Materials and Methods. Software analysis of cellular area and perimeter allows determination of cell roundness. Upon transfection of Bves-CT, an increase in roundness (white bar) of ≈25% is observed in comparison to cells transfected with GFP only (gray bar). Error bars represent SEM, significance determined by using standard Student's t test.