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. Author manuscript; available in PMC: 2008 Jul 19.
Published in final edited form as: Cell Death Differ. 2000 Dec;7(12):1244–1252. doi: 10.1038/sj.cdd.4400758

Figure 2. Sorting and propidium iodide staining of annexin V− and annexin V+ myeloma cell lines.

Figure 2

XG-6 cells were IL-6 starved for 24 hours and stained with FITC-annexin V. In the experiment outlined in the Figure, 40% of XG-6 cells were annexin V+. Annexin V+ and annexin V− cells were sorted using a FACS-Vantage cell sorter and DNA was labeled with PI. Results are the FACS profiles of annexin V staining of the unsorted and sorted populations as well as the profiles of DNA staining by PI. The percentages of annexin V+ cells or of cells with DNA content less than G1 are indicated in the panels.