TABLE 2.

Sequences of PCR primers and probes

Primer	Sequence	Probe	Sequence
Human genes
CYP2B6 Forward	5′-AAAGCGGAGTGTGGAGGA-3′	CYP2B6	FAM-5′-AGGAGGAG-3′-BHQ
Reverse	5′-AAGGTGGGGTCCATGAGG-3′
CYP2C9 Forward	5′-GTGCACGAGGTCCAGAGATAC-3′	CYP2C9	FAM-5′-CTTCTCCC-3′-BHQ
Reverse	5′-CAGGGAAATTAATATGGTTGTGC-3′
CYP2C19 Forward	5′-TGAAGGTGGAAATTTTAAGAAAAGTAA-3′	CYP2C19	FAM-5′-CAGCAGGA-3′-BHQ
Reverse	5′-CCCTCTCCCACACAAATCC-3′
CYP3A4 Forward	5′-CATGGACTTTTTAAGAAGCTTGG-3′	CYP3A4	FAM-5′-CTCTGCCT-3′-BHQ
Reverse	5′-TTCCATGTCAAACATACAAAAGC-3′
GAPDH Forward	5′-AGCCACATCGCTCAGACA-3′	GAPDH	FAM-5′-TGGGGAAG-3′-BHQ
Reverse	5′-GCCCAATACGACCAAATCC-3′
Mouse genes
Cyp2b10 Forward	5′-CAATGTTTAGTGGAGGAACTGCG-3′	Cyp2b10	FAM-5′-CCCAGGGAGCCCCCCTGGA-3′-TAMRA
Reverse	5′-CACTGGAAGAGGAACGTGGG-3′
GAPDH Forward	5′-CCAGAACATCATCCCTGCATC-3′	GAPDH	FAM-5′-CCGCCTGGAGAAACCTGCCAAGTATG-3′-TAMRA
Reverse	5′-GGTCCTCAGTGTAGCCCAAGAT-3′

FAM, fluorescein labeling; BHQ, black hole quencher; TAMRA, 5-carboxytetramethylrhodamine-labeled probe.