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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2008 Feb 2;109(1-2):96–107. doi: 10.1016/j.jsbmb.2007.12.013

Fig. 4. LPS+INFγ increases the expression of steroidogenic enzymes in cultured 1°MG.

Fig. 4

RT-PCR analysis of steroidogenic enzymes in cultured 1°MG. A–D) 1°MG cells were stimulated for 24hr with 100ng/ml LPS+ 10ng/ml INFγ (LPS), 10ng/ml INFγ (INFγ), LPS-conditioned media (LCM), 1mM db-cAMP (db-cAMP), or vehicle (Veh) (hatched bars). Bars represent the % expression of each enzyme with respect to vehicle and normalized to the housekeeping gene L27A. Values are the mean ± the SEM of 2–3 independent experiments done in triplicate. *, **, P < 0.05, P < 0.001 vs. Veh, respectively.