Table 1. Oligonucleotide Primers designed for RT-PCR Amplification of Steroidogenic Enzymes.
Primers for RT-PCR used to determine the expression of steroidogenic enzymes. The table shows the name of the protein analyzed (name of gene in parenthesis), specific oligonucleotide sequence, and the exons spanned by each amplicon.
| Protein(Gene) | Sequence (5′-3′) | Bases | Exons | |
|---|---|---|---|---|
| StAR | Fwd | GAGCTCTCTGCTTGGTTCTCAA | 218-239 | 2–3 |
| (Star) | Rev | TTGAGTATGCCCAAGGCCTT | 325-316 | |
| PBR | Fwd | TGCAGAAACCCTCTTGGCATC | 173-193 | 2–3 |
| (Tspo) | Rev | TGAAACCTCCCAGCTCTTTCC | 286-266 | |
| p450scc | Fwd | CCTATTCCGCTTTTCCTTTGAGTCC | 636-660 | 3–4 |
| (Cyp11a1) | Rev | CGCTCCCCAAATATAACACTGCTG | 686-663 | |
| p450c21 | Fwd | TGCCCCATCGTGCAACTAGG | 1064-1083 | 8–9 |
| (Cyp21a1) | Rev | AGCCGGAGATGCTGCTAGCC | 1102-1083 | |
| p450c17 | Fwd | TCGGCCCCAGATGGTGACTC | 416-435 | 1–2 |
| (Cyp17a1) | Rev | TGGTCCGACAAGAGGCCTAGAG | 454-433 | |
| Sts | Fwd | CCACTACTGCAACGCCTACCT | Isles, et al. 2004 | |
| (Sts) | Rev | CGTGAAGTAGAAGGCCTTCCA | Hum. Mol. Gen. | |
| Arsa | Fwd | TTCACTGCAGATAACGGTCCTG | 2038-2059 | 5–6 |
| (Arsa-1) | Rev | AGGAGTAATGTGACCTGGCCA | 2178-2158 | |
| Sulft | Fwd | ACAGCTCTTTCCAAGCCATGA | 614-634 | 5–6 |
| (Sult2a2) | Rev | TCCCCAGTTGTGCCTTTTCT | 722-703 | |
| 3βHSD1 | Fwd | TCTGAAAGGTACCCAGAACCTATTGG | 433-458 | 3–4 |
| (Hsd3b1) | Rev | TTGCTTGAACACAGGCCTCCA | 476-456 | |
| 3βHSD2 | Fwd | AAAGGTACCCAGAACTTATTGGAGGC | 417-442 | 3–4 |
| (Hsd3b2) | Rev | GGCACACTGGCTTGGATACAGG | 463-442 | |
| 3βHSD4 | Fwd | GGTCGAAAACAGGAAGAGGAATTGTC | 118-144 | 1–2 |
| (Hsd3b4) | Rev | TGGTCTTTGTCTGCAGCTTGGAC | 163-140 | |
| 17βHSD1 | Fwd | AGTGTGGGAGGCTTGATGGGA | 458-478 | 3–4 |
| (Hsd17b1) | Rev | CACTTCGTGGAATGGCAGTCC | 496-479 | |
| Arom | Fwd | CTTTGGAGAACAATTCGCCCTTTC | 418-441 | 3–4 |
| (Cyp19a1) | Rev | GCCCGTCAGAGCTTTCATAAAGAA | 462-439 | |
| 5αR | Fwd | TGTTTCCTGACAGGCTTTGCCC | 436-457 | 2–3 |
| (Srd5a1) | Rev | CCATGCCCACTAACCACAGGG | 475-455 | |
| 3αHSD | Fwd | GCCATCGTGAAAAACAATGG | 692-711 | 6–7 |
| (Hsd3a1) | Rev | AATCAGCGCAGGAGTTCGA | 795-777 | |
| L27A | Fwd | TGTTGGAGGTGCCTGTGTTCT | 442-462 | 1–1 |
| (Rpl27a) | Rev | CATGGAGAGAAGGAAGGATGC | 542-522 | |