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. 2008 Jun 18;3(6):e2427. doi: 10.1371/journal.pone.0002427

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Pattyn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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ISGylation pattern of FLAG-tagged Human, Chimpanzee, Rhesus Monkey, African Green Monkey and Mouse ISG15 in (A) human HekT cells, (B) monkey COS cells and (C) mouse N38 cells. Cells were only transfected with expression vectors for the indicated ISG15 orthologues, without extra stimulation. Upper panels: Western blot analysis on total cell lysates showing the total ISGylation pattern after ectopic expression of the indicated ISG15 orthologues as visualized by their FLAG-tag (anti-FLAG Ab). Lower panels: actin loading control (anti-actin Ab).