Skip to main content
. 2008 Jun 18;3(6):e2427. doi: 10.1371/journal.pone.0002427

Figure 3. AgmISG15, but not HuISG15, efficiently ISGylates UbcH10, H13 and H17.

Figure 3

V5-tagged UbcH7, H10, H13, H17 proteins were co-expressed in HekT cells with either FLAG-tagged Hu- or AgmISG15. (A) Total cell lysates were boiled in a SDS boiling buffer containing β-ME and loaded on a SDS-PAGE. The UbcH proteins were visualized by their V5-tag (Anti-V5 Ab). (B) Staining of blot (A) with anti-actin Ab as loading control. (C) Co-immunoprecipitation experiment with anti-FLAG affinity gel. The bound proteins were boiled in an SDS buffer containing reducing agents prior to separation by SDS PAGE. The immunoprecipitated UbcH proteins were visualized by an anti-V5 Ab.