Table 2. ISGylated targets identified by TAP purification and LC-MS/MS identification of AgmISG15 outnumber those of HuISG15.
Alternative name(s) Abbreviation | Isoforms? | HuISG15 | AgmISG15 | ||
Ub-Activating Enzyme | UbE1 | Uba | 0 | 15 | |
Ub-Conjugating Enzymes | UbcH6 | E2E1 | Q969T4, AQ96LR5 | 0 | 1 |
UbcH10 | E2C | 0 | 8 | ||
UbcH13 | E2N, bendless-like E2 | Q5JXB2 | 1 | 19 | |
UbcH16 | E2-25, HIP2 | 0 | 3 | ||
UbcH17 | E2T, HSPC150 | 0 | 1 | ||
Myosin light polypeptide 6B | P60660 | 0 | 1 | ||
Actin, cytoplasmic 1 | P60709,P62736 ,P63261 ,P63267 ,P68032 ,P68133 ,Q6S8J3 | ||||
0 | 1 | ||||
Phosphoglycerate mutase 2 | PGAM2 | P18669 | 0 | 1 | |
40S ribosomal protein S10 | RS10 | 0 | 1 | ||
Ubiquitin | 2 | 2 | |||
Heat Shock Proteins | HSP70-1 | HSP71 | lots, but on different peptides | 3 | 21 |
HSP70-5 | GRP78 | 2 | 1 | ||
HSP70-1L | HS71L | 0 | 1 | ||
HSP90 alpha | HSP86 | P08238 (276–284) | 0 | 1 | |
Heat shock cognate 71 kDa protein | HSP7C | 0 | 1 |
The numbers of MS/MS spectra identified with the different ISG15 orthologues are presented in the right columns.