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. 2008 Jun 25;3(6):e2476. doi: 10.1371/journal.pone.0002476

Figure 2. Butyrate transcriptionally up-regulates hPepT1 expression in Caco2-BBE cells.

Figure 2

Caco2-BBE cells were treated with 5 mM butyrate for 24 h and hPepT1 mRNA levels were assessed by A) semi-quantitative RT-PCR and B) real-time RT-PCR. Values represent means±S.E. of three determinations. **P<0.005 vs control. To examine butyrate effect on the stability of hPepT1 mRNA, cells were pre-incubated with 5 µg/ml Actinomycin D (AcD) for 30 min and then treated with butyrate for 24 h. C) Total RNA was analyzed by Northern blot using a probe specific to the hPepT1 transcript. RNA loading controls were shown as bottom panel. D) hPepT1 mRNA levels were quantified using real-time RT-PCR. Values expressed as normalized cycling threshold values relative to untreated (0 h) cells represent means±S.E. of three determinations.