Figure 11. Butyrate increases PepT1 expression and transport activity in vivo.

C57BL/6 mice were given regular water (H₂O) or water containing 5 mM butyrate (H₂O+Butyrate) for 24 h. Mice were then sacrificed and colons were removed. A) PepT1 mRNA level was assessed by real-time RT-PCR (n = 10/condition). B) PepT1 expression in colonic mucosa was analyzed by immunoblot (n = 10/condition) and representative PepT1 blots from different mice are shown. Expression of villin was shown as loading control. Bar graphs represent densitometric quantification of PepT1 bands. Values represent means±S.E. of four blots from independent experiments. C) Colonic apical membrane vesicles were prepared from H₂O− or H₂O+butyrate-treated mice (n = 25/condition) and analyzed for PepT1 expression by Western blot. D) PepT1-mediated specific uptake of [¹⁴C]Glycine-Sarcosine was measured in the colonic apical membrane vesicles (n = 25/condition). Values represent means±S.E. of three determinations. **P<0.005.