Figure 5. Non-phosphorylated peptide effectively competes with 32P-labeled MA11 peptide in binding to Caco2 cells.
Into each well containing Caco2 cells was added 0.005 µg 32P-labeled MA11 peptide and the indicated quantity of cold, non-phosphorylated MA11. After one hour of incubation, adherent cells were washed and the bound radioactive counts determined.